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Materials and Methods
Materials:
The Bags used for this experiment were prepared in LDPE & NYLON. All were of same & were for every product testing, Control Bags of LDPE were used for comparison.
Table-1 gives details of the food products tested in Efficacy Testing.
Table-1: Food products tested in Efficacy Testing.
No. | Product | Food Products |
1. | Freshness Plus Bags | Pomegranate |
2. | Lifeguard Bags | Mangoes |
3. | Halogen- CL Bags | Banana |
4. | Halogen-BR Bags | Banana |
5. | Halogen-IO Bags | Banana |
Methods:
- Quantitative Analysis –to check reduction in ethylene & carbon dioxide gas concentration in Bananas.
- Efficacy Testing – to study improvement in the shelf-life extension of fresh produce due to ethylene reduction.
- Quantitative Testing:
- Labeling of Test Samples (Table-2)
Table-2: Labelling of Test Products for Quantitative Analysis
No. Type of Product Labels 1. Control Control-LDPE 2. Control Control-NYLON 3. Freshness Plus Freshness Plus-LDPE 4. Freshness Plus Freshness Plus-NYLON 5. Food Fresh Food Fresh-LDPE 6. Food Fresh Food Fresh-NYLON 7. Anti-ripening CL Bags AR-CL-Micro AR-CL-Macro 8. Anti-ripening BR Bags AR-BR-Micro AR-BR-Macro 9. Anti-ripening IO Bags AR-IO-Micro AR-IO-Macro
- Labeling of Test Samples (Table-2)
Table-2: Labelling of Test Products for Quantitative Analysis
- Selection of Food Samples: Bananas were procured in semi-ripe form, no visual defects & with uniform maturity. Bananas were packed in perforated test films (0.5-0.7Kg per package) and stored at 20ºC.
- Ethylene Concentration: The reduction in ethylene concentration was measured over a period of 7 days.
- Carbon Dioxide Concentration: % CO2 content was measured in each test bag over a period of 7 days.
- Efficacy Testing:
- Selection of Food Samples: Fruits and vegetables were procured in semi-ripe stage, with no visual defects and of uniform maturity.
- Placement of Bags: 500 gms of each of the respective food products were placed in the respective bags.
- Observation Parameters: All sets were observed till the Control Sets were non-marketable with respect to- i. Percent physiological loss in weight (% PLW), ii. Visual changes in the food products through photographs and iii. Sensory evaluation using Ranking Test.
Results and Discussion
- . Quantitative Testing
- Ethylene Concentration

Fig 1: Ethylene Concentration in all Bags
- Carbon Dioxide Concentration
- Physiological Loss in Weight (% PLW)
- Freshness Plus Bags:

Fig 2: Carbon Dioxide Concentrations in all bags
Of all the Bags (Fig-2), the % CO2 in Food Fresh LDPE was the lowest at the end of 7 days followed by Food Fresh NYLON Bags. % CO2 in Freshness Plus NYLON & Halogen Micro Bags was at par at the end of the test period.

Fig 3: Physiological Weight loss in Pomegranate
-
- Lifeguard Bags:

Fig 4: Physiological Weight Loss in Mangoes
(Temperature 20.2- 26.8 ºC and 37-56 % RH)
As seen in Fig-3, at the end of 65 days, Freshness Plus showed only 6.82 % PLW as compared to 29.04 for Control.
- Anti-Ripening Halogen Micro Bags – As seen in Fig-5, PLW (%) of Bananas increased during storage but was found to be the lowest (0.90 %) when packed in AR-CL Micro Bags as against Control Bags (2.00 %) at the end of the storage period of 7 days.Fig-5 Physiological Weight

- Visual Observation with Photographs
- Freshness Plus Bags:

Control–Day 70

Freshness Plus–Day 70
Fig 6: Pomegranate in Freshness Plus
b. Lifeguard Bags:

Lifeguard-Day 17 | Control –Day 17


Fig 7: Mangoes in Lifeguard
Conclusions
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References:
- FAO, 2014 Website (fao.org).
- L. Raje, S. Sherlekar, K. Ramakrishnan, V.C.Malshe, G. Subbalakshmi, Post Harvest Preservation of mangoes by controlled release agents and adsorbent, Acta Horticulturae 455; 5th International Mango Symposium (1996).